Published March 24, 1989
| public
Journal Article
Direct measurements of sliding between outer doublet microtubules in swimming sperm flagella
- Creators
- Brokaw, Charles J.
Abstract
The relative motion of 40-nanometer gold beads bound to the exposed outer doublet microtubules of demembranated sea urchin sperm flagella has been observed and photographed during adenosine triphosphate (ATP)-reactivated swimming. This direct demonstration and measure of sliding displacements between outer doublet microtubules in actively bending flagella verifies the original sliding microtubule model for ciliary bending that was established by electron microscopy of fixed cilia and provides a new, functional measure for the diameter of the flagellar axoneme of 132 ± 8 nanometers.
Additional Information
© 1989 American Association for the Advancement of Science. Received 12 October 1988; accepted 12 January 1989. I thank S. M. Nagayama for laboratory assistance and M. Sheetz for the suggestion char uncoated gold beads should adhere to axonemal microtubules. Supported by NIH grants GM-18711 and RR-07003.Additional details
- Eprint ID
- 54145
- Resolver ID
- CaltechAUTHORS:20150127-132723793
- GM-18711
- NIH
- RR-07003
- NIH
- Created
-
2015-01-28Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field