Understanding dynamic changes in live cell adhesion with neutron reflectometry
Abstract
Neutron reflectometry (NR) was used to examine various live cells' adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell — surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment, e.g. for the development of targeted anti-invasive therapies.
Additional Information
© 2014 World Scientific Publishing Co. Received 10 October 2014; accepted 17 October 2014; published 10 December 2014. This work benefited from the use of the Lujan Neutron Scattering Center at LANSCE funded by the DOE Office of Basic Energy Sciences and Los Alamos National Laboratory under DOE Contract DE-AC52-06NA25396.Attached Files
Accepted Version - nihms658795.pdf
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Additional details
- PMCID
- PMC4334466
- Eprint ID
- 53998
- Resolver ID
- CaltechAUTHORS:20150122-132903375
- DE-AC52-06NA25396
- Department of Energy (DOE)
- Created
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2015-01-23Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field