The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes
Abstract
The neuron-restrictive silencer factor (NRSF) binds a DNA sequence element, called the neuron-restrictive silencer element (NRSE), that represses neuronal gene transcription in nonneuronal cells. Consensus NRSEs have been identified in 18 neuron-specific genes. Complementary DNA clones encoding a functional fragment of NRSF were isolated and found to encode a novel protein containing eight noncanonical zinc fingers. Expression of NRSF mRNA was detected in most nonneuronal tissues at several developmental stages. In the nervous system, NRSF mRNA was detected in undifferentiated neuronal progenitors, but not in differentiated neurons. NRSF represents the first example of a vertebrate silencer protein that potentially regulates a large battery of cell type-specific genes, and therefore may function as a master negative regulator of neurogenesis.
Additional Information
© 1995 American Association for the Advancement of Science. 31 October 1994: Accepted 1 February 1995. We thank T. Kadesch and P. Henthorn for cDNA libraries, N. Mori for HeLa nuclear extract, S. Padilla for technical assistance, S. Ou for antibody preparation, S. Tavtigian and B. Wold for cDNA libraries, L. Lo, A. Groves, and L. Banner for valuable advice M.-J. Fann and P. Patterson for actin probes, and B. Wold, L. Sommer, and S. Bultman for comments on the manuscript. Supported by NIH grant NS23476 and an Associate Investigatorship of the Howard Hughes Medical Institute (D.J.A.).Additional details
- Eprint ID
- 53947
- Resolver ID
- CaltechAUTHORS:20150121-121501631
- NS23476
- NIH
- Howard Hughes Medical Institute (HHMI)
- Created
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2015-01-21Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field