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Published February 7, 2007 | public
Journal Article

Non‐Canonical Amino Acids in Protein Polymer Design

Abstract

Protein polymers can be prepared with essentially absolute control of chain length, sequence, and stereochemistry through biological synthesis in microbial hosts. But for the polymer chemist, the twenty "canonical" amino acids provide an unacceptably small set of starting materials for macromolecular design. In the past several years, powerful techniques have been developed to introduce non-natural (non-canonical) amino acids containing a wide variety of functional groups into recombinant proteins. This review presents the methods currently available for the introduction of non-natural amino acids into engineered proteins both in vitro and in vivo. Methods for multiple- and single-site introduction are described. Recent applications of these methods are also addressed, particularly the development of biosensors, novel surfaces, and materials.

Additional Information

Copyright © Taylor & Francis Group, LLC Received 2 October 2006; Accepted 31 October 2006. Work on non-canonical amino acids at Caltech is supported by NIH grants GM62523 and EB1971, by the NSF Center for Science and Engineering of Materials, by the ARO Institute for Collaborative Biotechnologies, and by the Joseph J. Jacobs Institute for Molecular Engineering for Medicine.

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023