Sequence-specific cleavage of double helical DNA by triple helix formation
- Creators
- Moser, Heinz E.
-
Dervan, Peter B.
Abstract
Homopyrimidine oligodeoxyribonucleotides with EDTA-Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site. Oligonucleotides with EDTA.Fe at the 5' end cause a sequence specific double strand break. The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to the homopurine strand of Watson-Crick double helical DNA. The sequence-specific recognition of double helical DNA by homopyrimidine probes is sensitive to single base mismatches. Homopyrimidine probes equipped with DNA cleaving moieties could be useful tools for mapping chromosomes.
Additional Information
© 1987 American Association for the Advancement of Science. Received 8 July 1987; accepted 21 September 1987. Supported by grants from the National Institutes of Health (GM 35724) and the Ralph M. Parsons Foundation, and a fellowship (to H.E.M.) from the Swiss National Science Foundation.Attached Files
Published - 1700481.pdf
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Additional details
- Eprint ID
- 53137
- Resolver ID
- CaltechAUTHORS:20141223-091109973
- GM 35724
- NIH
- Ralph M. Parsons Foundation
- Swiss National Science Foundation (SNSF)
- Created
-
2014-12-23Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field