Evidence for a base-pairing interaction between U6 small nuclear RNA and the 5' splice site during the splicing reaction in yeast
- Creators
- Sawa, Hitoshi
- Abelson, John
Abstract
U6 small nuclear RNA (snRNA) is an essential factor in mRNA splicing. On the basis of the high conservation of its sequence, it has been proposed that U6 snRNA may function catalytically during the splicing reaction. If this is the case, it is likely that U6 snRNA interacts with the splice sites in the spliceosome to catalyze the reaction. We have used UV crosslinking to analyze the interactions of U6 snRNA with the splicing substrates during the yeast splicing reaction. Crosslinked products in which the central region of U6 snRNA was joined to the 5' splice site region of mRNA precursor and lariat intermediate were identified. The crosslinking sites were precisely located in one of these products. The results suggest a possible base-pairing interaction between U6 snRNA and the 5' splice site of the mRNA precursor.
Additional Information
© 1992 National Academy of Sciences. Contributed by John Abelson, August 21, 1992. We thank Suzanna Horvath and staff for synthesis of oligonucleotides, Y. Shimura for help in the early stage of this project, and C. Guthrie, E. Sontheimer, D. Wassarman, and members in our lab for critical comments on the manuscript. H.S. was supported by a long-term fellowship from the Human Frontier Science Program Organization. This work was supported by National Institutes of Health Grant GM 32637. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - PNAS-1992-Sawa-11269-73.pdf
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Additional details
- PMCID
- PMC50531
- Eprint ID
- 52894
- Resolver ID
- CaltechAUTHORS:20141216-144014556
- Human Frontier Science Program
- GM 32637
- NIH
- Created
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2014-12-16Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field