Strands hybridize in postreplicative adenovirus overlap recombination
Abstract
We describe a postreplicative mechanism for adenovirus overlap recombination. An adenovirus minichromosome system was used to study overlap recombination driven by adenovirus DNA replication. Crossing-over appeared to occur equally at, but not within, the borders of the overlap between partner molecules. We propose that recombination in the minichromosome system proceeds through an intermediate formed by direct hybridization of complementary sequences on displaced strands generated by adenovirus-specific DNA replication. Some, but not all, heterologous regions in the intermediate are susceptible to mismatch correction. This pathway is intrinsically nonreciprocal and differs significantly from other adenovirus recombinational mechanisms that have been described previously.
Additional Information
© 1991 National Academy of Sciences. Communicated by K. E. van Holde, October 4, 1990 (received for review September 1, 1990). We thank Dr. Kathleen L. Berkner for providing plasmid pIBIV used to make pXD6. This work was supported by National Institutes of Health Grant CA17699 (to G.D.P.) and American Cancer Society Grant PF-2938 (to K.G.A.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.Attached Files
Published - PNAS-1991-Ahern-105-9.pdf
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Additional details
- PMCID
- PMC50758
- Eprint ID
- 52520
- Resolver ID
- CaltechAUTHORS:20141210-075806932
- CA17699
- NIH
- PF-2938
- American Cancer Society
- Created
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2014-12-10Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field