The draft genome sequence of the ferret (Mustela putorius furo) facilitates study of human respiratory disease
Abstract
The domestic ferret (Mustela putorius furo) is an important animal model for multiple human respiratory diseases. It is considered the 'gold standard' for modeling human influenza virus infection and transmission. Here we describe the 2.41 Gb draft genome assembly of the domestic ferret, constituting 2.28 Gb of sequence plus gaps. We annotated 19,910 protein-coding genes on this assembly using RNA-seq data from 21 ferret tissues. We characterized the ferret host response to two influenza virus infections by RNA-seq analysis of 42 ferret samples from influenza time-course data and showed distinct signatures in ferret trachea and lung tissues specific to 1918 or 2009 human pandemic influenza virus infections. Using microarray data from 16 ferret samples reflecting cystic fibrosis disease progression, we showed that transcriptional changes in the CFTR-knockout ferret lung reflect pathways of early disease that cannot be readily studied in human infants with cystic fibrosis disease.
Additional Information
© 2014 Nature America, Inc. Received 20 May; accepted 22 October; published online 17 November 2014. This project was funded in whole or in part with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), Department of Health and Human Services, under Contract Nos. HHSN272200800060C and HHSN272201400005C and Public Health Service Grant P51OD010425 (M.G.K.). For the Broad Institute of MIT and Harvard, this project was funded in whole or in part with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272200900018C. D.J.G. also received training grants 9T32OD010423-06 and 5T32RR023916-05 from the NIH Office of the Director. J.F.E., S.R.T., X.S. and Y.Y. (University of Iowa) were supported under the NIH National Institute of Diabetes and Digestive and Kidney Diseases grants R37 DK047967, R24 DK096518 and P30 DK054759, and National Heart, Lung, and Blood Institute grant R01 HL108902. C.D. acknowledges support by SNSF advanced researcher fellowship (#136461). A.S. receives support under NIH National Institute of General Medical Sciences R01 GM102192. For S.S., B.A. and T.H., the Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a company registered in England with number 2742969. K.L.-T. also receives support under EURYI ERC. We thank Marshall Farms, New York, for providing three adult sable female ferrets (M. putorius furo; 421 days old).Attached Files
Published - nbt.3079.pdf
Supplemental Material - nbt.3079-S1.pdf
Supplemental Material - nbt.3079-S10.xlsx
Supplemental Material - nbt.3079-S11.xlsx
Supplemental Material - nbt.3079-S12.xlsx
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Supplemental Material - nbt.3079-S4.txt
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Supplemental Material - nbt.3079-S7.xlsx
Supplemental Material - nbt.3079-S8.xlsx
Supplemental Material - nbt.3079-S9.xlsx
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Additional details
- Eprint ID
- 52124
- Resolver ID
- CaltechAUTHORS:20141125-083439633
- National Institute of Allergy and Infectious Diseases
- NIH
- HHSN272200800060C
- Department of Health and Human Services
- HHSN272201400005C
- Department of Health and Human Services
- P51OD010425
- U. S. Public Health Service
- HHSN272200900018C
- Department of Health and Human Services
- 9T32OD010423-06
- NIH
- 5T32RR023916-05
- NIH
- R37 DK047967
- National Institute of Diabetes and Digestive and Kidney Diseases
- R24 DK096518
- National Institute of Diabetes and Digestive and Kidney Diseases
- P30 DK054759
- National Institute of Diabetes and Digestive and Kidney Diseases
- R01 HL108902
- National Heart, Lung, and Blood Institute
- 136461
- Swiss National Science Foundation (SNSF)
- R01 GM102192
- National Institute of General Medical Sciences
- EURYI ERC
- Created
-
2014-11-26Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field