TOPLESS Regulates Apical Embryonic Fate in Arabidopsis
Abstract
The embryos of seed plants develop with an apical shoot pole and a basal root pole. In Arabidopsis, the topless-1 (tpl-1) mutation transforms the shoot pole into a second root pole. Here, we show that TPL resembles known transcriptional corepressors and that tpl-1 acts as a dominant negative mutation for multiple TPL-related proteins. Mutations in the putative coactivator HISTONE ACETYLTRANSFERASE GNAT SUPERFAMILY1 suppress the tpl-1 phenotype. Mutations in HISTONE DEACETYLASE19, a putative corepressor, increase the penetrance of tpl-1 and display similar apical defects. These data point to a transcriptional repression mechanism that prevents root formation in the shoot pole during Arabidopsis embryogenesis.
Additional Information
© 2006 American Association for the Advancement of Science. Received 14 December 2005; accepted 27 April 2006. We thank L. Jones, J. Nemhauser, and F. Wellmer for critical reading of the manuscript. We also thank M. Hannon and K. Shively for assistance with the tpl loss-of-function alleles and D. B. Vert and N. Geldner for technical assistance. This work was supported by a Helen Hay Whitney postdoctoral fellowship and by NIH grants GM072764 (to J.A.L.) and GM45697 (to E.M.M.).Attached Files
Supplemental Material - Long.SOM.pdf
Files
| Name | Size | Download all |
|---|---|---|
|
md5:cc992718aeaf0859977a0fb75df2eb83
|
2.0 MB | Preview Download |
Additional details
- Eprint ID
- 51835
- DOI
- 10.1126/science.1123841
- Resolver ID
- CaltechAUTHORS:20141117-102347050
- Helen Hay Whitney Foundation
- GM072764
- NIH
- GM45697
- NIH
- Created
-
2014-11-17Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field