Two electrode platforms for DNA sensing based on DNA charge transport

Abstract

Recently, we have developed a new DNA array platform that is formed electrochem. and addressed using a secondary electrode. Electrochem. activation of a copper catalyst, patterned with one electrode, enables precise placement of different DNA sequences onto a second electrode surface. The two-electrode patterning and detection platform allows for both high spatial resoln. of the patterned DNA array and optimization of detection through DNA-mediated charge transport with electrocatalysis. These platforms have been used for the successful detection of single base mismatches, specific hybridization events, and sequence-specific binding of transcription factors. We describe the application of this two-electrode platform in the development of new cancer diagnostics.

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