A P1-based physical map of the region from D17S776 to D17S78 containing the breast cancer susceptibility gene BRCA1

Creators: Neuhausen, S. L.; Swensen, J.; Miki, Y.; Liu, Q.; Tavtigian, S.; Shattuck-Eidens, D.; Kamb, A.; Hobbs, M. R.; Gingrich, J.; Shizuya, H.; Kim, U.-J.; Cochran, C.; Futreal, P. A.; Wiseman, R. W.; Lynch, H. T.; Tonin, P.; Narod, S.; Cannon-Albright, L.; Skolnick, M. H.; Goldgar, D. E.

Abstract

BRCA1, a breast and ovarian cancer susceptibility locus, has been isolated and maps to 17q21. A physical map of the BRCA1, region which extended from the proximal boundary at D17S776 to the distal boundary at D17S78 was constructed and consists of 51 sequence tagged sites (STSs) from P1 and YAC ends, nine new short-tandem repeat (STR) polymorphic markers, and eight identified genes. The contig, which spans the estimated 2.3 Mb region, contains 29 P1s, 11 YACs, two BACs, and one cosmid. Based on key recombinants in two linked families, BRCA1 was further localized to a region bounded by D17S1321 on the proximal side and D17S1325 on the distal side. Within this estimated 600 kb region, the contig was composed completely of P1s and BACs ordered by STS-content mapping and confirmed by DNA restriction fragment fingerprinting.

Additional Information

© 1994 Oxford University Press. Received August 26, 1994; accepted September 28, 1994. We thank K. Fournier from Fred Hutchinson Cancer Research Center for supplying us with the somatic cell lines. We thank M. Ashton for technical assistance in drawing the figures and L.M. Bennen for confirmation of STS-content. We are grateful to D. Smoller and K. Schriewer from Genome Systems for their careful and efficient screening of the PI library. This work was supported by grants CA-48711 and CA-55914 from the National Institutes of Health, by Myriad Genetics, the National Cancer Institute of Canada Breast Cancer Initiative, the Canadian Genetics Diseases Network, and the Cedars Cancer Institute of the Royal Victoria Hospital.

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Resource type: Journal Article
Publisher: Oxford University Press
Published in: Human Molecular Genetics, 3(11), 1919-1926, ISSN: 0964-6906.