Published November 2013
| Supplemental Material
Journal Article
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Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas
Abstract
CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.
Additional Information
© 2013 Genetics Society of America. Manuscript received July 29, 2013; accepted for publication August 13, 2013; Early Online August 26, 2013. We thank Mihoko Kato and David Greenstein for helpful comments on the manuscript, participants in the Engineered Nucleases workshop at the 2013 International C. elegans meeting for collegial discussions, and WormBase. Highthroughput DNA sequencing was performed at the Millard and Muriel Jacobs Genetics and Genomics Laboratory at California Institute of Technology. P.W.S. is an investigator of the Howard Hughes Medical Institute, which supported this work.Attached Files
Supplemental Material - FileS1.pdf
Supplemental Material - SI.pdf
Supplemental Material - TableS1.pdf
Supplemental Material - TableS2.pdf
Supplemental Material - TableS3.pdf
Supplemental Material - TableS4.pdf
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Additional details
- PMCID
- PMC3813845
- Eprint ID
- 43810
- Resolver ID
- CaltechAUTHORS:20140213-100046738
- Howard Hughes Medical Institute (HHMI)
- Created
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2014-03-12Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field