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Published February 2014 | Supplemental Material + Published
Journal Article Open

Transcriptome analysis reveals novel players in the cranial neural crest gene regulatory network

Abstract

The neural crest is an embryonic stem cell population that gives rise to a multitude of derivatives. In particular, the cranial neural crest (CNC) is unique in its ability to contribute to both facial skeleton and peripheral ganglia. To gain further insight into the molecular underpinnings that distinguish the CNC from other embryonic tissues, we have utilized a CNC-specific enhancer as a tool to isolate a pure, region-specific NC subpopulation for transcriptional profiling. The resulting data set reveals previously unknown transcription factors and signaling pathways that may influence the CNC's ability to migrate and/or differentiate into unique derivatives. To elaborate on the CNC gene regulatory network, we evaluated the effects of knocking down known neural plate border genes and early neural crest specifier genes on selected neural crest-enriched transcripts. The results suggest that ETS1 and SOX9 may act as pan-neural crest regulators of the migratory CNC. Taken together, our analysis provides unprecedented characterization of the migratory CNC transcriptome and identifies new links in the gene regulatory network responsible for development of this critical cell population.

Additional Information

© 2014 Simões-Costa et al. Published by Cold Spring Harbor Laboratory Press. This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/. Received May 28, 2013; accepted in revised form November 6, 2013; Published in Advance January 3, 2014. We thank Diana Perez, Janice Grimm, and Rochelle Diamond for their excellent cell-sorting assistance. The Caltech Flow Cytometry Cell Sorting Facility is supported by funds from the Beckman Institute at Caltech. We thank Dr. Max Ezin for her help with live time-lapse imaging of enhancer activity, and Mike Stone and Brian Jun for technical assistance. This work was supported by NIH HD037105 and DE16459 (to M.E.B.) and the Pew Fellows Program in the Biomedical Sciences (to M.S.-C.), and a Caltech Cell Center fellowship from the Moore Foundation (to M.S.-C.).

Attached Files

Published - Genome_Res.-2014-Simões-Costa-281-90.pdf

Published - Simoes-Costa_2014.pdf

Supplemental Material - Supplemental_Figure1.jpg

Supplemental Material - Supplemental_Legends.docx

Supplemental Material - Supplemental_TSS_movie_vert.avi

Supplemental Material - Supplemental_Table1.xlsx

Supplemental Material - Supplemental_Table2.xlsx

Supplemental Material - Supplemental_Table3.xlsx

Supplemental Material - Supplemental_Table4.xlsx

Supplemental Material - Supplemental_Table5.xlsx

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Additional details

Created:
August 19, 2023
Modified:
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