Design and implementation of a biomolecular circuit for tracking protein concentration
Abstract
This paper describes the design, modeling, synthesis and preliminary validation of a protein concentration regulator circuit. The circuit is designed to maintain the level of a target protein to a reference level, specified by the amount of another protein. This is implemented using a single negative feedback loop that inhibits the production of the target protein once its concentration is equal to the reference amount. A mathematical model consisting of a set of ODEs is derived from mass action laws and Hill function approximations of protein production. Steady-state analysis of the model is used to predict parameter sensitivity and experimental behavior. We implemented this circuit in E. coli using scaffold-based sequestration and transcriptional activation. Preliminary experimental results show the system matching predictions from our model and performing the expected task.
Additional Information
© 2013 AACC. Research supported in part by the Benjamin M. Rosen Bioengineering Center, the California Institute of Technology, and the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. The authors would like to thank John E. Dueber and Weston R. Whitaker from the University of California, Berkeley, for providing the initial plasmid constructs, the E. coli strain WW62, and continuing experimental advice.Additional details
- Eprint ID
- 43088
- Resolver ID
- CaltechAUTHORS:20131219-095517199
- Benjamin M. Rosen Bioengineering Center
- Caltech
- W911NF-09-0001
- Army Research Office (ARO)
- Created
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2013-12-23Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field