The Establishment of Lysogenicity in Escherichia Coli
- Creators
- Lieb, Margaret
Abstract
When a population of sensitive bacteria is exposed to infection by a temperate phage, a large number of cells are not lysed and give rise to colonies containing lysogenic cells. Some of the factors influencing the probability that an infected cell of Escherichia coli, strain K12S, will give a lysogenic response are reported here. In addition, the present study deals with the change of free phage particles into the intracellular prophage form which is transmitted to all cells in a lysogenic culture. Information was obtained on specific questions regarding the establishment of lysogenicity, as, for example: When is the decision as to the type of response, i.e., lytic or lysogenic, made by the infected cell? How much time is required for transformation (reduction) of the infecting phage into prophage and what changes take place in the host cell? Is the prophage a cytoplasmic constituent of the lysogenic cell or linked to the genetic mechanism?
Additional Information
© 1953 American Society for Microbiology. Received for publication November 17, 1952. Aided by a fellowship from the National Foundation for Infantile Paralysis. The author wishes to express thanks to Dr. J. J. Weigle for his interest and help during the course of these investigations and also to Professors M. Delbrück and R. Dulbecco for their many valuable suggestions.Attached Files
Published - LIEjbact53.pdf
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Additional details
- PMCID
- PMC169593
- Eprint ID
- 43050
- Resolver ID
- CaltechAUTHORS:20131217-141305804
- National Foundation for Infantile Paralysis
- Created
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2013-12-23Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field