Recent advances in electron cryotomography and their application to imaging purified protein complexes, isolated organelles, and small cells

Abstract

A new 300kV, FEG, helium-cooled, energy-filtered "G2 Polara" electron cryoTEM was recently installed at Caltech. Using this instrument, we have now extensively tested the potential advantages of liquid helium cooling and the inclusion of two orthogonal tilt-series in the context of electron cryotomography. These results and the application of the technology to several specific biological problems are reported.

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