A Cocktail of Thermally Stable, Chemically Synthesized Capture Agents for the Efficient Detection of Anti-Gp41 Antibodies from Human Sera
Abstract
We report on a method to improve in vitro diagnostic assays that detect immune response, with specific application to HIV-1. The inherent polyclonal diversity of the humoral immune response was addressed by using sequential in situ click chemistry to develop a cocktail of peptide-based capture agents, the components of which were raised against different, representative anti-HIV antibodies that bind to a conserved epitope of the HIV-1 envelope protein gp41. The cocktail was used to detect anti-HIV-1 antibodies from a panel of sera collected from HIV-positive patients, with improved signal-to-noise ratio relative to the gold standard commercial recombinant protein antigen. The capture agents were stable when stored as a powder for two months at temperatures close to 60°C.
Additional Information
© 2013 Pfeilsticker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received June 14, 2013; Accepted August 13, 2013; Published October 7, 2013. This work was funded primarily by a grant from the Bill and Melinda Gates Foundation. Additional funding for the development of screening approaches, and for certain capture agent characterization methods, was provided by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. James R Heath is a founder and board member of Indi Molecular - InDi molecular was spun out of Integrated diagnostics recently, so a declaration of a competing interest with Indi Molecular is now more appropriate. Indi MOlecular is seeking to commercialize the PCC Agent technology. Bert T.Lai is an employee of Indi Molecular. Associated patents are pending. A patent has been filed (not issued) that is related to the capture agent development approach utilized in this paper (PCT/US09/47799/W02009155420). This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in our guide for authorsAttached Files
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Additional details
- PMCID
- PMC3792125
- Eprint ID
- 42158
- Resolver ID
- CaltechAUTHORS:20131031-134624016
- Bill and Melinda Gates Foundation
- W911NF-09-0001
- Army Research Office (ARO)
- Created
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2013-11-05Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field