Published June 27, 2014
| Submitted
Book Section - Chapter
Open
Designing libraries of chimeric proteins using SCHEMA recombination and RASPP
Abstract
SCHEMA is a method for designing libraries of novel proteins by recombination of homologous sequences. The goal is to maximize the number of folded proteins while simultaneously generating significant sequence diversity. Here, we use the RASPP algorithm to identify optimal SCHEMA designs for shuffling contiguous elements of sequence. To exemplify the method, SCHEMA is used to recombine five fungal cellobiohydrolases (CBH1s) to produce a library of more than 390,000 novel CBH1 sequences.
Additional Information
© 2014 Springer. The authors acknowledge funding from the Institute for Collaborative Biotechnologies through grant W911NF-09-D-0001 from the U.S. Army Research Office and The National Central University,Taiwan, through a Cooperative Agreement for Energy Research Collaboration. MAS is supported by a Resnick Sustainability Institute fellowship.Attached Files
Submitted - SCHEMA_MIMB.pdf
Files
SCHEMA_MIMB.pdf
Files
(166.3 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:9672a559b04238673a8bb77d2986e73f
|
166.3 kB | Preview Download |
Additional details
- Eprint ID
- 41910
- Resolver ID
- CaltechAUTHORS:20131015-081653940
- W911NF-09-D-001
- Army Research Office (ARO)
- National Central University (Taiwan)
- Resnick Sustainability Institute
- Created
-
2013-10-15Created from EPrint's datestamp field
- Updated
-
2021-11-10Created from EPrint's last_modified field
- Caltech groups
- Resnick Sustainability Institute
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 1179