Published July 2013
| Accepted Version
Journal Article
Open
The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards
Abstract
The apparent handedness of an EM-tomography reconstruction depends on a number of conventions and can be confused in many ways. As the number of different hardware and software combinations being used for electron tomography continue to climb, and the reconstructions being produced reach higher and higher resolutions, the need to verify the hand of the results has increased. Here we enumerate various steps in a typical tomography experiment that affect handedness and show that DNA origami gold nanoparticle helices can be used as convenient and fail-safe handedness standards.
Additional Information
© 2013 Elsevier Inc. Received 11 October 2012; Received in revised form 17 April 2013; Accepted 19 April 2013; Available online 30 April 2013. We thank Tim Liedl and Robert Schreiber for the DNA origami gold nanoparticle helices, Mark S. Ladinsky for negative stain sample preparation and cryosectioning, Paul Rothemund for helpful advice and discussions, Lu Gan and Alasdair McDowall for helpful discussions and technical support. We thank Julio Ortiz, Friedrich Förster, John Heumann and Martin Beck for helpful communication. This work was supported in part by the Beckman Institute at Caltech as well as NIH grants R01 GM094800B and 2P50GM082545-06 to GJJ and P41GM103431 to A. Hoenger.Attached Files
Accepted Version - nihms507321.pdf
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nihms507321.pdf
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Additional details
- PMCID
- PMC3765063
- Eprint ID
- 40905
- Resolver ID
- CaltechAUTHORS:20130822-102116618
- Caltech Beckman Institute
- R01 GM094800B
- NIH
- 2P50GM082545-06
- NIH
- P41GM103431
- NIH
- Created
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2013-08-23Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field