Characterization of the local temperature in space and time around a developing Drosophila embryo in a microfluidic device
Abstract
This paper characterizes a microfluidic platform that differentially controls the temperature of each half of a living Drosophila melanogaster fruitfly embryo in space and time (E. M. Lucchetta, J. H. Lee, L. A. Fu, N. H. Patel and R. F. Ismagilov, Nature, 2005, 434, 1134-1138). This platform relies on laminar flow of two streams of liquid with different temperature, and on rapid prototyping in polydimethylsiloxane (PDMS). Here, we characterized fluid flow and heat transport in this platform both experimentally and by numerical simulation, and estimated the temperature distribution around and within the embryo by numerical simulation, to identify the conditions for creating a sharper temperature difference (temperature step) over the embryo. Embryos were removed from the device and immunostained histochemically for detection of Paired protein. Biochemical processes are sensitive to small differences in environmental temperature. The microfluidic platform characterized here could prove useful in understanding dynamics of biochemical networks as they respond to changes in temperature.
Additional Information
© Royal Society of Chemistry 2006. Received 14th November 2005, Accepted 16th December 2005. First published on the web 12th January 2006. We thank Nipam H. Patel (Department of Integrative Biology, Molecular and Cell Biology, and Howard Hughes Medical Institute, University of California–Berkeley, Berkeley, CA 94720-3140, USA) for helpful discussions and for providing DP311 antibody. This work was supported by the Searle Scholars Program, the NSF MRSEC Program under DMR-0213745, and the MRSEC microfluidic facility funded by the NSF.Attached Files
Published - b516119c.pdf
Supplemental Material - Ismagilov_loc_Lucchetta_2006_6_185_embryo_local_temperature_characterization.pdf
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Additional details
- Eprint ID
- 40832
- Resolver ID
- CaltechAUTHORS:20130821-160725195
- Searle Scholars Program
- DMR-0213745
- NSF
- Created
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2013-08-27Created from EPrint's datestamp field
- Updated
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2021-11-10Created from EPrint's last_modified field