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Published May 2013 | public
Journal Article

Enhancer and gene traps for molecular imaging and genetic analysis in zebrafish

Abstract

Enhancer and gene trapping methods are highly effective means for the identification and functional analysis of transcriptionally active genes. With recent advances in fluorescent proteins and transposon based integration technologies, a growing family of trapping approaches has been developed in zebrafish, offering powerful tools to both visualize and functionally dissect gene networks during development. Coupled with the intrinsic advantages of the zebrafish model system, creative genetic engineering of trap vectors has enabled high-resolution molecular imaging and genetic manipulations. This review highlights the different enhancer and gene trap approaches that have been developed in zebrafish and offers insights into the strengths, limitations and experimental strategies for their application to enrich our knowledge of gene function and the cellular processes they control.

Additional Information

© 2013 The Authors. Development, Growth & Differentiation © 2013 Japanese Society of Developmental Biologists. Received 10 January 2013; revised 4 March 2013; accepted 5 March 2013. Article first published online: 8 Apr. 2013. We thank Rusty Lansford and Masahiro Kitano for helpful comments. Work related to gene trapping in our laboratory has been supported by NHGRI Center of Excellence in Genomic Science grant P50HG004071.

Additional details

Created:
August 19, 2023
Modified:
October 24, 2023