Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published January 29, 2013 | Published
Journal Article Open

Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy

Abstract

Two-photon light sheet microscopy combines nonlinear excitation with the novel sheet-illumination, orthogonal to the detection direction, to achieve high penetration depth, high acquisition speed, and low photodamage, compared with conventional imaging techniques. These advantages allow unprecedented observation of the processes that govern embryogenesis, where the ability to image fast the dynamic three dimensional structure of the developing embryo, over extended periods of time, is critical. We present a selection of applications where two-photon light sheet microscopy is utilized to observe the dynamics of proteins, cells, and tissues, toward an understanding of the construction program of the developing embryos.

Additional Information

© 2013 Biophysical Society. Published by Elsevier Inc.

Attached Files

Published - 1-s2.0-S0006349512031189-main.pdf

Files

1-s2.0-S0006349512031189-main.pdf
Files (42.2 kB)
Name Size Download all
md5:abdc950b7b4aa82925f220c28f44106c
42.2 kB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 23, 2023