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Published March 1, 2013 | Accepted Version + Supplemental Material
Journal Article Open

Diversification of oral and aboral mesodermal regulatory states in pregastrular sea urchin embryos

Abstract

Specification of the non-skeletogenic mesoderm (NSM) in sea urchin embryos depends on Delta signaling. Signal reception leads to expression of regulatory genes that later contribute to the aboral NSM regulatory state. In oral NSM, this is replaced by a distinct oral regulatory state in consequence of Nodal signaling. Through regulome wide analysis we identify the homeobox gene not as an immediate Nodal target. not expression in NSM causes extinction of the aboral regulatory state in the oral NSM, and expression of a new suite of regulatory genes. All NSM specific regulatory genes are henceforth expressed exclusively, in oral or aboral domains, presaging the mesodermal cell types that will emerge. We have analyzed the regulatory linkages within the aboral NSM gene regulatory network. A linchpin of this network is gataE which as we show is a direct Gcm target and part of a feedback loop locking down the aboral regulatory state.

Additional Information

© 2013 Elsevier Inc. Received 30 August 2012. Received in revised form 29 November 2012. Accepted 29 November 2012. Available online 19 December 2012. We would like to thank Jongmin Nam for his help with Nanotag experiments and Gilson Sanchez for help with in situ stainings. Many thanks to Cynthia Solek and Jonathan Rast for sharing unpublished data. We are grateful for many discussions with Joel Smith and Celina Juliano. Research was supported by NIH grant HD037105 and by the Lucille P. Markey Charitable Trust.

Attached Files

Accepted Version - nihms-437601.pdf

Supplemental Material - 1-s2.0-S0012160612006525-mmc1.jpg

Supplemental Material - 1-s2.0-S0012160612006525-mmc2.jpg

Supplemental Material - 1-s2.0-S0012160612006525-mmc3.jpg

Supplemental Material - mmc5.xlsx

Supplemental Material - mmc6.xlsx

Supplemental Material - mmc7.xlsx

Supplemental Material - mmc8.xlsx

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