Evidence for dynamic rearrangements but lack of fate or position restrictions in premigratory avian trunk neural crest
Abstract
Neural crest (NC) cells emerge from the dorsal trunk neural tube (NT) and migrate ventrally to colonize neuronal derivatives, as well as dorsolaterally to form melanocytes. Here, we test whether different dorsoventral levels in the NT have similar or differential ability to contribute to NC cells and their derivatives. To this end, we precisely labeled NT precursors at specific dorsoventral levels of the chick NT using fluorescent dyes and a photoconvertible fluorescent protein. NT and NC cell dynamics were then examined in vivo and in slice culture using two-photon and confocal time-lapse imaging. The results show that NC precursors undergo dynamic rearrangements within the neuroepithelium, yielding an overall ventral to dorsal movement toward the midline of the NT, where they exit in a stochastic manner to populate multiple derivatives. No differences were noted in the ability of precursors from different dorsoventral levels of the NT to contribute to NC derivatives, with the exception of sympathetic ganglia, which appeared to be 'filled' by the first population to emigrate. Rather than restricted developmental potential, however, this is probably due to a matter of timing.
Additional Information
© 2013 Published by The Company of Biologists Ltd. Accepted November 30, 2012. Posted online before print January 14, 2013. Gene expression profiling samples were analyzed by the Fluidigm Genetic Analysis Facility of the Molecular Genetics Core Facility at Children's Hospital Boston. Gap43-TagRFP was kindly prepared by DRI and H2B-eGFP was a kind gift from Robb Krumlauf. We thank Connie Gonzalez for technical assistance with in situ hybridization. Funding: P.K. was partially funded by the National Institutes of Health (NIH) [NIH-1R01HD057922] and the Stowers Institute for Medical Research. M.E.B. was partially funded [HD037105 and DE16459]. The Molecular Genetics Core Facility at Children's Hospital Boston is supported by the NIH [NIH-P30-HD18655]. Deposited in PMC for release after 12 months. Competing interests statement: The authors declare no competing financial interests.Attached Files
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Additional details
- PMCID
- PMC3557777
- Eprint ID
- 37248
- Resolver ID
- CaltechAUTHORS:20130301-114905448
- NIH-1R01HD057922
- NIH
- HD037105
- NIH
- DE16459
- NIH
- Stowers Institute for Medical Research
- NIH-P30-HD18655
- NIH
- Created
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2013-03-01Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field