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Published October 15, 2012 | Published + Supplemental Material
Journal Article Open

A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole

Abstract

The cell poles constitute key subcellular domains that are often critical for motility, chemotaxis, and chromosome segregation in rod-shaped bacteria. However, in nearly all rods, the processes that underlie the formation, recognition, and perpetuation of the polar domains are largely unknown. Here, in Vibrio cholerae, we identified HubP (hub of the pole), a polar transmembrane protein conserved in all vibrios, that anchors three ParA-like ATPases to the cell poles and, through them, controls polar localization of the chromosome origin, the chemotactic machinery, and the flagellum. In the absence of HubP, oriCI is not targeted to the cell poles, chemotaxis is impaired, and a small but increased fraction of cells produces multiple, rather than single, flagella. Distinct cytoplasmic domains within HubP are required for polar targeting of the three ATPases, while a periplasmic portion of HubP is required for its localization. HubP partially relocalizes from the poles to the mid-cell prior to cell division, thereby enabling perpetuation of the polar domain in future daughter cells. Thus, a single polar hub is instrumental for establishing polar identity and organization.

Additional Information

© 2012 by Cold Spring Harbor Laboratory Press. Received June 29, 2012; revised version accepted August 27, 2012. These authors contributed equally to this work. We are grateful to Janet Iwasa for making Figure 6D, Paula Montero Llopis for an expert tutorial in Microbe Tracker, and John Mekalanos for providing the mapped transposon library. We also thank Richard Losick, Hubert Lam, and Waldor laboratory members for helpful discussions and comments on the manuscript. S.R. was funded with a post-doctoral fellowship from the Villum Kann Rasmussen Foundation. This work was supported by the NIAID R37 AI-042347 (to M.K.W.), NIGMS R01 GM094800B (to G.J.J), and HHMI (to M.K.W. and G.J.J.).

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Published - Genes_Dev.-2012-Yamaichi-2348-60.pdf

Supplemental Material - MovieS1.mov

Supplemental Material - MovieS2.mov

Supplemental Material - Supplement_3_4.doc

Supplemental Material - sup_figs.pdf

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August 22, 2023
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