Unusual Gene Order and Organization of the Sea Urchin Hox Cluster
Abstract
While the highly consistent gene order and axial colinear patterns of expression seem to be a feature of vertebrate hox gene clusters, this pattern may be less well conserved across the rest of the bilaterians. We report the first deuterostome instance of an intact hox cluster with a unique gene order where the paralog groups are not expressed in a sequential manner. The finished sequence from BAC clones from the genome of the sea urchin, Strongylocentrotus purpuratus, reveals a gene order wherein the anterior genes (Hox1, Hox2 and Hox3) lie nearest the posterior genes in the cluster such that the most 3′ gene is Hox5. (The gene order is 5′-Hox1, 2, 3, 11/13c, 11/13b, 11/13a, 9/10, 8, 7, 6, 5-3′.) The finished sequence result is corroborated by restriction mapping evidence and BAC-end scaffold analyses. Comparisons with a putative ancestral deuterostome Hox gene cluster suggest that the rearrangements leading to the sea urchin gene order were many and complex.
Additional Information
© 2005 Wiley-Liss, Inc. Issue published online: 10 Jan 2006; Article first published online: 22 Aug 2005; Manuscript Accepted: 11 Jul 2005; Manuscript Received: 10 May 2005. We acknowledge support by the National Institutes of Health Grant HD05753 (to E.H.D.), the National Science Foundation Grant IBN9604454 (to R.A.C.) and the Beckman Institute, California Institute of Technology (PI: E.H.D.). A portion of this work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program and by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory under contract No. DE-AC03- 76SF00098 and Los Alamos National Laboratory under contract No. W-7405-ENG-36. Grant sponsor: NIH; Grant number: HD05753; Grant sponsor: NSF; Grant number: IBN9604454; Grant sponsor: Beckman Institute, California Institute of Technology; Grant sponsor: DoE; Grant number: LLNL W-7405-Eng-48; Grant sponsor: DoE; Grant number: LBNL DE-AC03-76SF00098; Grant sponsor: DoE; Grant number: LANL W-7405-ENG-36. R. Andrew Cameron and Lee Rowen contributed equally to this work.Additional details
- Eprint ID
- 35081
- DOI
- 10.1002/jez.b.21070
- Resolver ID
- CaltechAUTHORS:20121024-162724677
- HD-05753
- NIH
- IBN9604454
- NSF
- Caltech Beckman Institute
- W-7405-ENG-48
- Department of Energy (DOE)
- DE-AC03-76SF00098
- Department of Energy (DOE)
- W-7405-ENG-36
- Department of Energy (DOE)
- Created
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2012-10-25Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field