Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published June 1987 | public
Journal Article

Correct Cell-Type-Specific Expression of a Fusion Gene Injected into Sea Urchin Eggs

Abstract

A fusion gene construct containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of CyIIIa actin gene regulatory sequences was injected into unfertilized eggs of the sea urchin Strongylocentrotus purpuratus, and early pluteus stage embryos that developed from these eggs were fixed and sectioned for analysis by in situ hybridization. A [^3H]RNA antisense probe for CAT mRNA was hybridized to 5-μm embryo sections. Autoradiographic signal denoting the presence of CAT mRNA was detected only over aboral ectoderm cells, in which the CyIIIa gene is normally expressed, and not over any recognizable regions of gut or oral ectoderm included in the same sections.

Additional Information

© 1987 Academic Press, Inc. Received September 22, 1986; accepted in revised form December 29, 1986. We thank R. C. Angerer and K. H. Cox for helpful consultations on the techniques of in situ hybridization. This research was supported by NIH Grant HD05753. R.R.F. was supported by an NIH postdoctoral training grant (HD07257), R.A.C. by NSF Grant DCB-8544707.

Additional details

Created:
August 19, 2023
Modified:
October 19, 2023