Published 2012
| public
Book Section - Chapter
A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids
- Creators
- Hess, Sonja
- Others:
- Alterman, Michail A.
- Hunziker, Peter
Abstract
The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences, including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields. To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here, we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents, such as Marfey's reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers.
Additional Information
© 2012 Springer Science+Business Media, LLC. This work was supported by the Intramural Program of the National Institute of Diabetes, Digestive and Kidney Diseases, Z01 DK070004-04, the Beckman Institute and the Betty and Gordon Moore Foundation.Additional details
- Eprint ID
- 34143
- DOI
- 10.1007/978-1-61779-445-2_7
- Resolver ID
- CaltechAUTHORS:20120917-112748155
- Z01 DK070004-04
- National Institute of Diabetes, Digestive and Kidney Diseases
- Caltech Beckman Institute
- Gordon and Betty Moore Foundation
- Created
-
2012-09-17Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 828