Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published 2012 | public
Book Section - Chapter

A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids

Abstract

The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences, including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields. To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here, we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents, such as Marfey's reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers.

Additional Information

© 2012 Springer Science+Business Media, LLC. This work was supported by the Intramural Program of the National Institute of Diabetes, Digestive and Kidney Diseases, Z01 DK070004-04, the Beckman Institute and the Betty and Gordon Moore Foundation.

Additional details

Created:
August 19, 2023
Modified:
January 13, 2024