Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published June 1968 | Published
Journal Article Open

Stimulation of φX174 production in mitomycin C-treated Escherichia coli cells by caffeine

Abstract

After pretreatment of an Escherichia coli C culture with mitomycin C, the deoxyribonucleic acid (DNA) synthesis of the bacteria, as well as that of infecting phage φX174, is very largely inhibited. Although the bacterial DNA synthesis is similarly inhibited by mitomycin C in uvr^(-)mutants (P. Howard-Flanders, R. P. Boyce, and L. Theriot, Genetics 53:1119, 1966), phage DNA synthesis and progeny formation take place in a normal manner (B. H. Lindqvist and R. L. Sinsheimer, J. Mol. Biol. 30:69, 1967). The reason for this behavior of the uvr^- cells is not understood. Since it is known that uvr^+ cells do not reactivate double-stranded ultraviolet-damaged phage DNA in the presence of adequate concentrations of caffeine (W. Sauerbier, Biochem. Biophys. Res. Commun. 14:340, 1964), it was of interest to test the ability of mitomycin C-treated uvr^+ cells to produce phage under these conditions.

Additional Information

© 1968 American Society for Microbiology. Received for publication 28 February 1968. One of us (H.S.) was supported by a NATO fellowship. This research was supported by Public Health Service grant GM13554 from the National Institute of General Medical Sciences.

Attached Files

Published - STEjvir68.pdf

Files

STEjvir68.pdf
Files (151.7 kB)
Name Size Download all
md5:0ad3ff29b46588526d53f7fc4375ee4a
151.7 kB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023