Published October 25, 1973
| public
Journal Article
Determination of Intracellular pH by ^(31)P Magnetic Resonance
- Creators
- Moon, Richard B.
- Richards, John H.
Abstract
Observation of the ^(31)P signal from various intracellular phosphates can provide a convenient, nondestructive technique for determining intracellular conditions such as pH. This procedure has been explored with particular reference to the erythrocyte. Both the chemical shift of intracellular inorganic phosphate relative to that of serum phosphate and the positions of, and more especially the difference between, the chemical shifts of 2,3-diphosphoglycerate have been used to monitor intracellular pH of erythrocytes whose hemoglobin has been liganded with carbon monoxide.
Additional Information
© 1973 American Society of Biological Chemists, Inc. Received for publication, July 5, 1973. This work was supported by Grants NIHL 15198 and NHIL 15162 from the United States Public Health Service. This is Contribution No. 4725 from the Church Laboratory of Chemical Biology.Additional details
- Eprint ID
- 33175
- Resolver ID
- CaltechAUTHORS:20120814-130208418
- NIHL 15198
- Public Health Service
- NIHL 15162
- Public Health Service
- Created
-
2012-08-14Created from EPrint's datestamp field
- Updated
-
2019-10-03Created from EPrint's last_modified field
- Other Numbering System Name
- Caltech Church Laboratory of Chemical Biology
- Other Numbering System Identifier
- 4725