Published July 2012
| Supplemental Material + Published + Accepted Version
Journal Article
Open
Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures
Abstract
We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.
Additional Information
© 2012 American Chemical Society. Received: April 20, 2012. Revised: June 2, 2012. Publication Date (Web): June 5, 2012. We thank A. Fernandez and M. Boitano for assistance in preparing ZMW arrays. Supported by a Beckman Fellowship and an NIH NRSA to C.I.R., by a California Tobacco-Related Disease Research Fellowship to R.S., and by the US National Institutes of Health (NS34407).Attached Files
Published - Richards2012p19101Nano_Lett.pdf
Accepted Version - nihms-383248.pdf
Supplemental Material - nl301480h_si_001.pdf
Files
Richards2012p19101Nano_Lett.pdf
Additional details
- PMCID
- PMC3397148
- Eprint ID
- 33128
- Resolver ID
- CaltechAUTHORS:20120813-111734004
- Arnold and Mabel Beckman Foundation
- NIH Predoctoral Fellowship
- California Tobacco-Related Disease Research Program
- NS34407
- NIH
- Created
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2012-08-13Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field