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Published May 1974 | Published
Journal Article Open

Electron Microscope Analysis of Partial Denaturation of F Factor Deoxyribonucleic Acid

Hsu, Ming-Ta

Abstract

Partial denaturation pattern of sex factor deoxyribonucleic acid of Escherichia coli was studied by electron microscopy. Clustering of the adenine-plusthymine-rich regions in one part of the molecule was revealed. The positions of these regions were located on the physical map of F by analyzing the partial denaturation pattern of heteroduplexes between F and F-prime factors with various parts of F sequences deleted.

Additional Information

© 1974 American Society for Microbiology. Received for publication 28 January 1974. I am deeply grateful to Norman Davidson for advice and counsel, and for support through Public Health Service grant GM 10991 from the National Institute of General Medical Sciences. This work was initiated during a discussion with Donald Robberson. His generosity in providing advice and unpublished data are greatly appreciated.

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