Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published November 25, 1982 | public
Journal Article

Expression of an Abelson murine leukemia virus-encoded protein in Escherichia coli causes extensive phosphorylation of tyrosine residues

Abstract

A segment of the Abelson murine leukemia virus (A-MuLV) genome was inserted into an Escherichia coli plasmid designed to allow the expression of the protein encoded by the viral gene. Bacteria expressing the A-MuLV-encoded protein were isolated; they had new phosphorylated proteins in which the phosphate was linked to tyrosine residues. These proteins included many that must be E. coli protein. One phosphotyrosine-containing protein of 62,000 molecular weight had reactivity with antiserum specific for authentic A-MuLV protein. The A-MuLV protein thus appears to be a tyrosine-specific protein kinase which is active in E. coli.

Additional Information

© 1982 American Society for Biochemistry and Molecular Biology. Received for publication, June 7, 1982. This work was supported by Grant MV-34N from the American Cancer Society, Grants CA26717 and CA14051 (core grant to S. E. Luria) from the National Cancer Institute, and a contribution from the Whitehead Charitable Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Postdoctoral fellow of the Jane Coffin Childs Memorial Fund for Medical Research. Postdoctoral fellow of the National Institutes of Health.

Additional details

Created:
August 19, 2023
Modified:
October 18, 2023