Advances in multiphoton microscopy for imaging embryos
Abstract
Multiphoton imaging is a promising approach for addressing current issues in systems biology and high-content investigation of embryonic development. Recent advances in multiphoton microscopy, including light-sheet illumination, optimized laser scanning, adaptive and label-free strategies, open new opportunities for embryo imaging. However, the literature is often unclear about which microscopy technique is most adapted for achieving specific experimental goals. In this review, we describe and discuss the key concepts of imaging speed, imaging depth, photodamage, and nonlinear contrast mechanisms in the context of recent advances in live embryo imaging. We illustrate the potentials of these new imaging approaches with a selection of recent applications in developmental biology.
Additional Information
© 2011 Elsevier Ltd. Available online 13th September 2011. This review comes from a themed issue on Developmental mechanisms, patterning and evolution Edited by Sean Megason, Shankar Srinivas, Mary Dickinson and Anna-Katerina Hadjantonakis. We thank members of the Laboratory for Optics and Biosciences at Ecole Polytechnique (Palaiseau, France) and of Scott E. Fraser group at Caltech (Pasadena, CA, USA) for fruitful discussions. T.V.T. is supported by the NIH (U01 DE020063). This work was supported by ANR (ANR-2010-JCJC-1510-01) and the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 268379 (into to Morphogenesis project) to W.S.Additional details
- Eprint ID
- 28793
- DOI
- 10.1016/j.gde.2011.08.003
- Resolver ID
- CaltechAUTHORS:20120113-131125278
- U01 DE020063
- NIH
- ANR-2010-JCJC-1510-01
- ANR
- 268379
- European Union Seventh Framework Programme (FP7/2007-2013)
- Created
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2012-01-13Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field