Activated chemoreceptor arrays remain intact and hexagonally packed
Abstract
Bacterial chemoreceptors cluster into exquisitively sensitive, tunable, highly ordered, polar arrays. While these arrays serve as paradigms of cell signalling in general, it remains unclear what conformational changes transduce signals from the periplasmic tips, where attractants and repellents bind, to the cytoplasmic signalling domains. Conflicting reports support and contest the hypothesis that activation causes large changes in the packing arrangement of the arrays, up to and including their complete disassembly. Using electron cryotomography, here we show that in Caulobacter crescentus, chemoreceptor arrays in cells grown in different media and immediately after exposure to the attractant galactose all exhibit the same 12 nm hexagonal packing arrangement, array size and other structural parameters. ΔcheB and ΔcheR mutants mimicking attractant- or repellent-bound states prior to adaptation also show the same lattice structure. We conclude that signal transduction and amplification must be accomplished through only small, nanoscale conformational changes.
Additional Information
© 2011 Blackwell Publishing Ltd. Accepted 14 September, 2011. Article first published online: 12 Oct 2011. This work was supported in part by the Howard Hughes Medical Institute and by gifts to Caltech from the Gordon and Betty Moore Foundation.Attached Files
Accepted Version - nihms457996.pdf
Supplemental Material - MMI_7854_sm_BriegelFigure1.tif
Supplemental Material - MMI_7854_sm_BriegelFigure3.tif
Supplemental Material - MMI_7854_sm_BriegelFigure4.tif
Supplemental Material - MMI_7854_sm_Briegelfigure2.tif
Supplemental Material - MMI_7854_sm_movie1WT.mp4
Supplemental Material - MMI_7854_sm_movie2WT.mp4
Supplemental Material - MMI_7854_sm_movie3deltaCheR.mp4
Supplemental Material - MMI_7854_sm_movie4deltaCHeB.mp4
Supplemental Material - MMI_7854_sm_movie5deltaCheB.mp4
Supplemental Material - MMI_7854_sm_movie6deltaCheR.mp4
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Additional details
- PMCID
- PMC3641884
- Eprint ID
- 28518
- DOI
- 10.1111/j.1365-2958.2011.07854.x
- Resolver ID
- CaltechAUTHORS:20111219-115630715
- Howard Hughes Medical Institute (HHMI)
- Gordon and Betty Moore Foundation
- Created
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2011-12-20Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field