Published 2012
| Accepted Version
Book Section - Chapter
Open
Microarray Method for the Rapid Detection of Glycosaminoglycan–Protein Interactions
- Creators
- Rogers, Claude J.
-
Hsieh-Wilson, Linda C.
- Other:
- Chevolot, Yann
Abstract
Glycosaminoglycans (GAGs) perform numerous vital functions within the body. As major components of the extracellular matrix, these polysaccharides participate in a diverse array of cell-signaling events. We have developed a simple microarray assay for the evaluation of protein binding to various GAG subclasses. In a single experiment, the binding to all members of the GAG family can be rapidly determined, giving insight into the relative specificity of the interactions and the importance of specific sulfation motifs. The arrays are facile to prepare from commercially available materials.
Additional Information
© 2012 Springer Science+Business Media, LLC. We thank Dr. Igor Antoshechkin, Director of the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology, and Dr. Jose Luis Riechmann for assistance with printing the microarrays and Joshua M. Brown, Russell E. Roberson, and Dr. Igor Antoshechkin for critically reading the manuscript. This work was supported by the National Institutes of Health (R01 GM093627).Attached Files
Accepted Version - nihms338488.pdf
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Additional details
- PMCID
- PMC4137757
- Eprint ID
- 27763
- DOI
- 10.1007/978-1-61779-373-8_22
- Resolver ID
- CaltechAUTHORS:20111114-080659427
- R01 GM093627
- NIH
- Created
-
2011-11-21Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 808