Published September 19, 2011
| Accepted Version
Journal Article
Open
A BODIPY-Cyclooctyne for Protein Imaging in Live Cells
Abstract
Cellular proteins that bear reactive azides can be imaged by fluorescence microscopy following strain-promoted ligation to cyclooctyne dyes. Here we describe BODIPY-cyclooctyne (BDPY), a membrane-permeant fluorophore that can be used to label intracellular proteins in live mammalian cells. Flow cytometry reveals fluorescence signals more than 25-fold above background after labeling of azide-tagged cells with BDPY.
Additional Information
© 2011 Wiley-VCH Verlag. Received: May 1, 2011. Published online on August 9, 2011. We thank Julie Liu, Matthew J. Hangauer, Jeremy M. Baskin, Michael S. Boyce, Andrew Mehle, and Carolyn R. Bertozzi for valuable discussions. We are grateful to Mandy K. Vink for preparing Aha and to Stacy A. Maskarinec for providing Rat-1 fibroblasts. Marissa L. Mock, Ying Ying Lu, and Rebecca E. Connor made helpful comments on the manuscript. Imaging was done at the Biological Imaging Center (Beckman Institute at Caltech). Flow cytometry was done at Caltech under the guidance of Diana Perez and Rochelle Diamond. Support was provided by a Fannie and John Hertz Foundation fellowship to K.E.B. , by an NIH postdoctoral fellowship (F32M071214) to J.D.F., and by grants from the NIH (R01M062523) and the Army Research Office (W911NF0810227).Attached Files
Accepted Version - nihms381687.pdf
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Additional details
- PMCID
- PMC3387918
- Eprint ID
- 27354
- Resolver ID
- CaltechAUTHORS:20111021-134710184
- Fannie and John Hertz Foundation
- F32M071214
- NIH Postdoctoral Fellowship
- R01M062523
- NIH
- W911NF0810227
- Army Research Office (ARO)
- Created
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2011-10-21Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field