Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published April 2004 | Published
Journal Article Open

Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae

Abstract

The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we report the isolation of Bbp1p as a polo-box interacting protein by a yeast two-hybrid screen. Bbp1p localizes to the periphery of the central plaque of the SPB and plays an important role in SPB duplication. Similarly, Cdc5p localized to the cytoplasmic periphery of the SPB. In vitro binding studies showed that Cdc5p interacted with the N-terminal domain of Bbp1p (Bbp1pΔC), but apparently not with Mps2p, a component shown to form a stable complex with Bbp1p. In addition, Bbp1p, but likely not Mps2p, was required for proper localization of Cdc5p to the SPB. The C-terminal coiled-coil domain of Bbp1p (Bbp1p^(243–385)), which is crucial for both the homodimerization and the SPB localization, could target the localization-defective Cdc5pΔC to the SPB and induce the release of Cdc14p from the nucleolus. Consistent with this observation, expression of CDC5ΔC-BBP1^(243–385) under CDC5 promoter control partially complemented the cdc5Δ defect. These data suggest that Bbp1pΔC interacts with the polo-box domain of Cdc5p, and this interaction is critical for the subcellular localization and mitotic functions of Cdc5p.

Additional Information

© 2004 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0). Submitted July 2, 2003; Revised November 7, 2003; Accepted November 21, 2003. Published online before print February 6, 2004. Monitoring Editor: Tim Stearns. We are grateful to Doug Kellogg, John Kilmartin, Kim Nasmyth, Elmar Schiebel, Akio Sugino, and Akio Toh-e for strains. We also thank Geum-Yi Kim, Young-Wook Cho, and Satoshi Asano for technical support, and Susan Garfield for helping with confocal microscopy. This work was supported in part by National Institutes of Health grant R01 GM-51312 (to M.W.).

Attached Files

Published - PARmbc04.pdf

Files

PARmbc04.pdf
Files (1.2 MB)
Name Size Download all
md5:2dfb9566b2261aadf23b8cfb23e8a13c
1.2 MB Preview Download

Additional details

Created:
August 19, 2023
Modified:
October 24, 2023