Live imaging of endogenous Collapsin response mediator protein-1 expression at subcellular resolution during zebrafish nervous system development
Abstract
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins that are functionally important during vertebrate development. We have generated a zebrafish gene trap line that produces fluorescently tagged Crmp1 protein, which can be dynamically tracked in living fish at subcellular resolution. The results show that Crmp1 is expressed in numerous sites in the developing nervous system. Early expression is apparent in the forebrain, epiphysis, optic tectum and the developing spinal cord. In the larval brain, Crmp1 is expressed in several distinct brain regions, such as the telencephalon, habenula and cerebellum. In addition, it is expressed in the spinal cord in a manner that persists in the larva. The results suggest that this Crmp1 protein trap line offers a powerful tool to track selected neuronal populations at high resolution.
Additional Information
© 2011 Elsevier B.V. Received 2 March 2011; revised 16 May 2011; Accepted 17 May 2011. Available online 24 May 2011. We wish to thank the following people: Ho-Yin Leung for performing 3' RACE and LeighAnn Fletcher for technical help in the Caltech Centers of Excellence in Genomic Science fish facility. This work is supported by USPHS P50HG004071.Attached Files
Accepted Version - nihms298668.pdf
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Additional details
- PMCID
- PMC3163798
- Eprint ID
- 27119
- DOI
- 10.1016/j.gep.2011.05.002
- Resolver ID
- CaltechAUTHORS:20111007-100356026
- P50HG004071
- NIH
- Created
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2011-10-07Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field