A Micro Device for Separation of Erythrocytes and Leukocytes in Human Blood
- Creators
- Zheng, Siyang
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Tai, Yu-Chong
- Kasdan, Harvey
Abstract
We report a MEMS device for continuous and binary separation of leukocytes (white blood cells, WBCs) and erythrocytes (red blood cells, RBCs) by their sizes. The system consists of PDMS cylindrical pillars inside fluidic chamber on glass slides. A 420 µm lateral separation was achieved for 5 µm and 10 µm beads. The critical particle size for separation was successful predicted by simulation and experimentally determined by calibration with polystyrene beads. The separation function (lateral displacement versus particle size) was also measured experimentally. Diluted whole blood and blood fraction of concentrated leukocytes were tested with the devices with erythrocyte to leukocyte ratio in agreement with blood count results. Experiments with fluorescent labeled leukocytes confirm the separation of leukocytes from erythrocytes and measured the separation efficiency.
Additional Information
© 2005 IEEE. Issue Date: 17-18 Jan. 2006. Date of Current Version: 10 April 2006. This work is supported by NASA through National Space Biomedical Research Institute (NSBRI). The cooperative agreement number is NCC 9-58-317.Attached Files
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Additional details
- Eprint ID
- 25141
- Resolver ID
- CaltechAUTHORS:20110829-095853628
- NCC 9-58-317
- NASA
- Created
-
2011-08-31Created from EPrint's datestamp field
- Updated
-
2021-11-09Created from EPrint's last_modified field
- Series Name
- Proceedings of Annual International Conference of the IEEE Engineering in Medicine and Biology Society