Published October 2007
| public
Journal Article
Electron Cryotomography
- Creators
- Murphy, Gavin E.
-
Jensen, Grant J.
Abstract
Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required instrumentation, sample preparation and limitations, data collection, typical results, and future prospects are summarized briefly.
Additional Information
© 2007 BioTechniques. We would like to thank Wolfgang Baumeister for allowing us to re-use Figure 1; Giovanni Cardone and Alasdair Steven for Figure 2E; and Ping Zhu, Ken Taylor and Kenneth Roux for Figure 2, F–H. This work was supported in part by NIH grants no. P01 GM66521 and R01 AI067548 to G.J.J., DOE grant no. DE-FG02-04ER63785 to G.J.J., a Searle Scholar Award to G.J.J., NIH graduate fellowship F31 EB 004179 to G.E.M., and gifts to Caltech from the Ralph M. Parsons Foundation, the Agouron Institute, and the Gordon and Betty Moore Foundation.Additional details
- Eprint ID
- 24669
- Resolver ID
- CaltechAUTHORS:20110803-161436056
- PO1 GM66521
- NIH
- R01 AI067548
- NIH
- DE-FG02-04ER63785
- Department of Energy (DOE)
- Searle Scholar Award
- F31 EB 004179
- NIH
- Ralph M. Parsons Foundation
- Agouron Institute
- Gordon and Betty Moore Foundation
- Created
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2011-08-04Created from EPrint's datestamp field
- Updated
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2019-10-03Created from EPrint's last_modified field