Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates
Abstract
A general method of rigid, specific labeling of proteins with gold clusters has been devised. The method relies on the conjugation of a glutathione monolayer-protected gold cluster (MPC) with a single chain Fv antibody fragment (scFv), mutated to present an exposed cysteine residue. Efficient formation of a gold−thiolate bond between the MPC and scFv depends on activation of the gold cluster by chemical oxidation. Once formed, the MPC−scFv conjugate is treated with a reductant to quench cluster reactivity. The procedure has been performed with an MPC with an average Au71 core and an scFv directed against a tetrameric protein, the influenza neuraminidase. A complex of the MPC−scFv conjugate with the neuraminidase was isolated, and the presence of four gold clusters was verified by cryoelectron microscopy.
Additional Information
© 2006 American Chemical Society. Received August 28, 2005. Publication Date (Web): February 2, 2006. We thank Christopher Chidsey for many helpful discussions. pGC-NC10 was a kind gift from Olan Dolezal. C.J.A. received support from NIH training grant T32 GM0829. This research was supported by NIH grants AI21144 and NIH GM63025.Attached Files
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Additional details
- Eprint ID
- 24645
- DOI
- 10.1021/ja0555668
- Resolver ID
- CaltechAUTHORS:20110803-092706578
- T32 GM0829
- NIH Predoctoral Fellowship
- AI21144
- NIH
- GM63025
- NIH
- Created
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2011-08-03Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field