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Published July 2011 | public
Journal Article

Dynamic Lineage Analysis of Embryonic Morphogenesis Using Transgenic Quail and 4D Multispectral Imaging

Abstract

We describe the development of transgenic quail that express various fluorescent proteins in targeted manners and their use as a model system that integrates advanced imaging approaches with conventional and emerging molecular genetics technologies. We also review the progression and complications of past fate mapping techniques that led us to generate transgenic quail, which permit dynamic imaging of amniote embryogenesis with unprecedented subcellular resolution.

Additional Information

© 2011 Wiley-Liss, Inc. Received 30 December 2010; Revised 27 March 2011; Accepted 28 March 2011. Article first published online: 17 Jun 2011. Contract grant sponsor: NIH CEGS, NHGRI; Contract grant number: P50HG004071; Contract grant sponsor: NIH NIDCR FaceBase; Contract grant number: U01 DE020063 Y.S. was a postdoctoral fellow supported by the Japan Society for the Promotion of Science (JSPS). We thank Scott E. Fraser for helpful comments on the figures and David Huss for excellent bird husbandry.

Additional details

Created:
August 22, 2023
Modified:
October 23, 2023