Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published July 2011 | Supplemental Material + Published
Journal Article Open

Toward high-content/high-throughput imaging and analysis of embryonic morphogenesis

Abstract

In vivo study of embryonic morphogenesis tremendously benefits from recent advances in live microscopy and computational analyses. Quantitative and automated investigation of morphogenetic processes opens the field to high-content and high-throughput strategies. Following experimental workflow currently developed in cell biology, we identify the key challenges for applying such strategies in developmental biology. We review the recent progress in embryo preparation and manipulation, live imaging, data registration, image segmentation, feature computation, and data mining dedicated to the study of embryonic morphogenesis. We discuss a selection of pioneering studies that tackled the current methodological bottlenecks and illustrated the investigation of morphogenetic processes in vivo using quantitative and automated imaging and analysis of hundreds or thousands of cells simultaneously, paving the way for high-content/high-throughput strategies and systems analysis of embryonic morphogenesis.

Additional Information

© 2011 Wiley-Liss, Inc. Received 18 December 2010; Revised 8 April 2011; Accepted 14 April 2011. Article first published online: 24 Jun. 2011. Contract grant sponsor: NIH - Center of Excellence in Genomic Science, Contract grant number: P50 HG004071, Contract grant sponsor: Beckman Institute, Contract grant sponsor: Marie Curie International Reintegration Grant within the 7th European Community Framework Programme, Contract grant sponsor: Agence Nationale de la Recherche (Programme Jeunes Chercheuses/Jeunes Chercheurs). The authors thank the members of Scott E. Fraser and Angelike Stathopoulos laboratories at Caltech (Pasadena, CA, USA) and of Jérôme Collignon's team at Institute Jacques Monod (Paris, France) for fruitful discussions.

Attached Files

Published - Truong2011p15372Genesis.pdf

Supplemental Material - DVG_20760_sm_SuppInfo.pdf

Supplemental Material - DVG_20760_sm_suppinfomovie1.mov

Files

DVG_20760_sm_SuppInfo.pdf
Files (7.8 MB)
Name Size Download all
md5:b34b5a527151d94e29bb9285fc0a2a94
7.3 MB Download
md5:d5471bb36f6f7190a0dd78c368dcb251
49.0 kB Preview Download
md5:06ec3b0b2aefe5d3cb1147335eb24f79
495.6 kB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 23, 2023