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Published March 2011 | public
Journal Article

Dual-excitation-wavelength fluorescence spectra and elastic scattering for differentiation of single airborne pollen and fungal particles

Abstract

Fluorescence spectra of aerosolized pollen (12 species or subspecies) and fungal (4 species) samples measured using a Dual-excitation-wavelength Particle Fluorescence Spectrometer (DPFS) are reported. The DPFS records two fluorescence spectra and two elastic scattering intensities from single airborne particles as they flow through an optical cell. Each particle is illuminated sequentially, first with a pulse from a 263-nm wavelength laser, followed by a second pulse from a 351-nm laser. The two dispersed fluorescence spectra (from 280 nme700 nm) and elastic scattering intensities are measured by a single 32-anode photomultiplier tube. Principal component analysis (PCA) of the spectra is used to examine differences in the spectra. Plots of several principal components show that samples of pollens and fungal materials can largely be differentiated. These preliminary results suggest that fluorescence spectra and elastic scattering may be useful for real-time discrimination among a variety of airborne pollens, fungal materials, and other airborne particles.

Additional Information

© 2010 Elsevier Ltd. Received 20 August 2010; revised 10 December 2010; accepted 17 December 2010. Available online 8 January 2011. This research was supported by the U.S. Army Research Laboratory mission funds.

Additional details

Created:
August 22, 2023
Modified:
October 23, 2023