Proteomic analysis of polyketide and nonribosomal peptide biosynthesis
- Creators
- Meier, Jordan L.
- Burkart, Michael D.
Abstract
Polyketides and non-ribosomal peptides are in a class of natural products important both as drug sources and as dangerous toxins and virulence factors. While studies over the last two decades have provided substantial characterization of the modular synthases that produce these compounds at the genetic level, their understanding at the protein level is much less understood. New proteomic platforms called an orthogonal active site identification system (OASIS) and proteomic interrogation of secondary metabolism (PrISM) have been developed to identify and quantify natural product synthase enzymes. Reviewed here, these tools offer the means to discover and analyze modular synthetic pathways that are limited by genetic techniques, opening the tools of contemporary proteomics to natural product sciences.
Additional Information
© 2010 Elsevier Ltd. Available online 17 November 2010. This review comes from a themed issue on Omics. Edited by Kate Carroll and Pieter Dorrestein. This work was supported by a National Institutes of Health EUREKA Award (GM094924).Additional details
- Eprint ID
- 23206
- Resolver ID
- CaltechAUTHORS:20110401-113808707
- GM094924
- NIH EUREKA Award
- Created
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2011-04-01Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field