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Published 2006 | Published
Book Section - Chapter Open

Nonuniform temporal alignment of slice sequences for four-dimensional imaging of cyclically deforming embryonic structures

Abstract

The temporal alignment of nongated slice-sequences acquired at different axial positions in the living embryonic zebrafish heart permits the reconstruction of dynamic, three-dimensional data. This approach overcomes the current acquisition- speed limitation of confocal microscopes for real-time three-dimensional imaging of fast processes. Current synchronization methods align and uniformly scale the data in time, but do not compensate for slight variations in the heart rhythm that occur within a heartbeat. Therefore, they impose constraints on the admissible data quality. Here, we derive a nonuniform registration procedure based on the minimization of the absolute value of the intensity difference between adjacent slice-sequence pairs. The method compensates for temporal intra-sample variations and allows the processing of a wider range of data to build functional, dynamic models of the beating embryonic heart. We show reconstructions from data acquired in living, fluorescent zebrafish embryos.

Additional Information

© 2006 IEEE. Issue Date: 6-9 April 2006; Date of Current Version: 08 May 2006. We thank Sean Megason, Le Trinh, and Chris Waters for help with zebrafish preparation and advice on imaging techniques. M.L. would like to thank Michael Unser for helpful discussions and for sharing unpublished work on related topics.

Attached Files

Published - Liebling2006p89842007_4Th_Ieee_International_Symposium_On_Biomedical_Imaging_Macro_To_Nano_Vols_1-3.pdf

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Liebling2006p89842007_4Th_Ieee_International_Symposium_On_Biomedical_Imaging_Macro_To_Nano_Vols_1-3.pdf

Additional details

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August 19, 2023
Modified:
January 13, 2024