Fully automated, sequential tilt-series acquisition with Leginon
Abstract
Electron tomography has become a uniquely powerful tool for investigating the structures of individual cells, viruses, and macromolecules. Data collection is, however, time consuming and requires expensive instruments. To optimize productivity, we have incorporated one of the existing tilt-series acquisition programs, UCSF Tomo, into the well-developed automatic electron microscopy data collection package Leginon to enable fully automatic, sequential tilt-series acquisition. Here we describe how UCSF Tomo was integrated into Leginon, what users must do to set up a data collection session, how the automatic collection proceeds, how archived data about the process can be accessed and used, and how the software has been tested.
Additional Information
© 2009 Elsevier Inc. Received 21 October 2008; revised 27 March 2009; accepted 31 March 2009. Available online 8 April 2009. We thank Drs. Bill Tivol, Prabha Dias, Jane Ding, Lu Gan, Ariane Briegel, Elizabeth Wright, Zhou Li and Alasdair McDowall for advice and testing during the development of MSI Tomography. This work was supported in part by NIH Grants R01 AI067548 and P50 GM082545 and DOE Grant DE-FG02-04ER63785 to GJJ, a Searle Scholar Award to GJJ, the Beckman Institute at Caltech, and gifts to Caltech from the Gordon and Betty Moore Foundation and Agouron Institute. Leginon development at the National Resource for Automated Molecular Microscopy is supported by the National Institutes of Health though the National Center for Research Resources' P41 program (RR17573 and R01 RR023093).Attached Files
Accepted Version - nihms-123659.pdf
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Additional details
- PMCID
- PMC2724967
- Eprint ID
- 21267
- Resolver ID
- CaltechAUTHORS:20101209-110311836
- R01 AI067548
- NIH
- P50 GM082545
- NIH
- DE-FG02-04ER63785
- Department of Energy (DOE)
- Searle Scholars Program
- Caltech Beckman Institute
- Gordon and Betty Moore Foundation
- Agouron Institute
- RR17573
- NIH
- R01 RR023093
- NIH
- Created
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2010-12-14Created from EPrint's datestamp field
- Updated
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2021-11-09Created from EPrint's last_modified field