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Published November 1, 2009 | Accepted Version + Supplemental Material
Journal Article Open

Myosin-X is critical for migratory ability of Xenopus cranial neural crest cells

Abstract

The neural crest is a highly migratory cell population, unique to vertebrates, that forms much of the craniofacial skeleton and peripheral nervous system. In exploring the cell biological basis underlying this behavior, we have identified an unconventional myosin, myosin-X (Myo10) that is required for neural crest migration. Myo10 is highly expressed in both premigratory and migrating cranial neural crest (CNC) cells in Xenopus embryos. Disrupting Myo10 expression using antisense morpholino oligonucleotides leads to impaired neural crest migration and subsequent cartilage formation, but only a slight delay in induction. In vivo grafting experiments reveal that Myo10-depleted CNC cells migrate a shorter distance and fail to segregate into distinct migratory streams. Finally, in vitro cultures and cell dissociation–reaggregation assays suggest that Myo10 may be critical for cell protrusion and cell–cell adhesion. These results demonstrate an essential role for Myo10 in normal cranial neural crest migration and suggest a link to cell–cell interactions and formation of processes.

Additional Information

© 2009 Published by Elsevier Inc. Received 11 February 2009; revised 18 August 2009; accepted 18 August 2009. Available online 25 August 2009. We thank Dr. William M. Bement for providing us full-length Xenopus Myo10 constructs, Dr. Dominique Alfandari for providing protocols for grafting experiment, Dr. Chenbei Chang for providing Wnt3a and Noggin constructs, and Dr. Ken Cho for providing microarray analysis tool. This work was supported by NS36585 and HD037105 to MBF.

Attached Files

Accepted Version - nihms-568059.pdf

Supplemental Material - Nie2009p6278Dev_Biol_sup1.mov

Supplemental Material - Nie2009p6278Dev_Biol_sup2.mov

Supplemental Material - Nie2009p6278Dev_Biol_sup3.mov

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August 21, 2023
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