The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo
Abstract
Maternal and zygotic activities of the homeodomain protein PAL-1 specify the identity and maintain the development of the multipotent C blastomere lineage in the C. elegans embryo. To identify PAL-1 regulatory target genes, we used microarrays to compare transcript abundance in wild-type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomeres. pal-1-dependent C-lineage expression was verified for select candidate target genes by reporter gene analysis, though many of the target genes are expressed in additional lineages as well. The set of validated target genes includes 12 transcription factors, an uncharacterized wingless ligand and five uncharacterized genes. Phenotypic analysis demonstrates that the identified PAL-1 target genes affect specification, differentiation and morphogenesis of C-lineage cells. In particular, we show that cell fate-specific genes (or tissue identity genes) and a posterior HOX gene are activated in lineage-specific fashion. Transcription of targets is initiated in four temporal phases, which together with their spatial expression patterns leads to a model of the regulatory network specified by PAL-1.
Additional Information
© The Company of Biologists Ltd 2005. Accepted 3 February 2005. First published online 16 March 2005. Strain JK3363 was kindly provided by Judith Kimble. We thank Shai Shen-Orr for bioinformatics support. This work was funded by an NIH GM64429 to C.P.H. Supplementary material for this article is available at http://dev.biologists.org/cgi/content/full/132/8/1843/DC1Attached Files
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Additional details
- Eprint ID
- 15903
- Resolver ID
- CaltechAUTHORS:20090917-100109169
- GM64429
- NIH
- Created
-
2009-09-22Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field